Evaluation of reactive oxygen species generating AirOcare system for reducing airborne microbial populations in a meat processing plant

The microbial contamination of meat and meat products is of continuing concern to the meat industry and regulatory agencies. Air has been established as a source of microbial contamination in slaughter and processing facilities. The objective of this research was to determine the efficacy of reactive oxygen species (ROS) generating AirOcare equipment in reducing airborne bacteria in a meat processing environment. Bacterial strains found in ground beef were used to artificially contaminate the air using a 6jet Collison nebulizer. Airborne bacterial populations in the meat processing room were monitored every 24 h at multiple locations using a Staplex 6 stage air sampler. Total aerobic, Gram-negative, and lactic acid bacterial populations were determined by sampling on R2A agar, MacConkey agar and Lactobacilli MRS agar, respectively. Approximately 3 log reductions of lactic acid bacteria and Gram-negative bacteria were observed after 24 h of treatment (p \ 0.05) compared to *1.5 log reduction in the control treatment. Further exposure with ROS significantly reduced lactic acid bacteria and Gram-negative bacteria in the air at 48 and 96 h sampling intervals. These findings reveal that reactive oxygen species treatment using AirOcare unit significantly reduces airborne contamination in a meat processing environment.